HIV-1 Infection of T Cells and Macrophages Are Differentially Modulated by Virion-Associated Hck: A Nef-Dependent Phenomenon

HIV-1 Infection of T Cells and Macrophages Are Differentially Modulated by Virion-Associated Hck: A Nef-Dependent Phenomenon

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The proline repeat motif (PxxP) of Nef is required for interaction with the SH3 domains of macrophage-specific Src kinase Hck.However, the implication of this interaction for viral replication and infectivity in macrophages and T lymphocytes remains unclear.Experiments in HIV-1 infected macrophages confirmed Cutters the presence of a Nef:Hck complex which was dependent on the Nef proline repeat motif.The proline repeat motif of Nef also enhanced both HIV-1 infection and replication in macrophages, and was required for incorporation of Hck into viral particles.Unexpectedly, wild-type Hck inhibited infection of macrophages, but Hck was shown to enhance infection of primary T lymphocytes.

These results indicate that the interaction between Nef and Hck is important for Nef-dependent modulation of viral infectivity.Hck-dependent enhancement Performance Shirts of HIV-1 infection of T cells suggests that Nef-Hck interaction may contribute to the spread of HIV-1 infection from macrophages to T cells by modulating events in the producer cell, virion and target cell.